Are Specific Gene Expressions of Extracellular Matrix and Nucleus Pulposus Affected by Primary Cell Cultures Prepared from Intact or Degenerative Intervertebral Disc Tissues?

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dc.authoridakyuva, yener/0000-0001-8171-5929
dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337
dc.authoridYilmaz, Ibrahim/0000-0003-1503-4046
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorYasar Sirin, Duygu
dc.contributor.authorKaplan, Necati
dc.contributor.authorAkyuva, Yener
dc.contributor.authorGonultas, Aylin
dc.date.accessioned2025-03-26T17:35:09Z
dc.date.available2025-03-26T17:35:09Z
dc.date.issued2019
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractAIM: To determine the gene expression patterns of nucleus pulposus (NP) in cell cultures obtained from degenerated or intact tissues. MATERIAL and METHODS: Whereas 12 of the cases were diagnosed with lumbar disc herniation and had undergone lumbar microdiscectomy, 12 cases had undergone traumatic intervertebral discectomy and corpectomy, along with discectomy after spinal trauma. NP-specific markers and gene expressions of the reagents of the extracellular matrix in the experimental setup were tested at the 0th, 24th, and 48th hours by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Visual evaluations were simultaneously made in all samples using invert and fluorescence microscopy. Vitality and proliferation analyses were evaluated by UV spectrophotometer. As a method of statistical evaluation, Spearman was used for categorical variants, and the Pearson correlation was used for variants with numerical and plain distribution. RESULTS: No association was found either between the tissue type and times (r=0.000; p=1.000) or between the region that the tissue was obtained from and hypoxia transcription factor-1 alpha (HIF-1 alpha) gene expression (r=0.098; p=0.245). There was no correlation between cell proliferation and chondroadherin (CHAD) expression or between type II collagen (COL2A1) and CHAD gene expressions. It was found that CHAD and HIF-1 alpha gene expressions and HIF-1 alpha and COL2A1 gene expressions affected cell proliferation. CONCLUSION: Cell culture setups are of paramount importance because they may influence the pattern of changes in the gene expressions of the cells used in these setups.
dc.identifier.doi10.5137/1019-5149.JTN.22210-17.2
dc.identifier.endpage52
dc.identifier.issn1019-5149
dc.identifier.issue1
dc.identifier.pmid29484626
dc.identifier.scopus2-s2.0-85053427602
dc.identifier.scopusqualityQ3
dc.identifier.startpage43
dc.identifier.trdizinid355638
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.22210-17.2
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/355638
dc.identifier.urihttps://hdl.handle.net/20.500.14704/1060
dc.identifier.volume29
dc.identifier.wosWOS:000454722700007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250326
dc.subjectAnnulus fibrosus; Degenerative disc disease; Nucleus pulposus; Specific marker; Pharmaco-molecular therapy; Human primary cell culture
dc.titleAre Specific Gene Expressions of Extracellular Matrix and Nucleus Pulposus Affected by Primary Cell Cultures Prepared from Intact or Degenerative Intervertebral Disc Tissues?
dc.typeArticle

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