Does insulin, transferrin and selenous acid preparation effect chondrocyte proliferation?

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dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337
dc.authoridYilmaz, Ibrahim/0000-0003-1503-4046
dc.contributor.authorGokce, Alper
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorGokay, Nevzat Selim
dc.contributor.authorCan, Levent
dc.contributor.authorGokce, Cigdem
dc.date.accessioned2025-03-26T17:34:44Z
dc.date.available2025-03-26T17:34:44Z
dc.date.issued2014
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractObjective: The aim of this study was to test the hypothesis that insulin, human transferrin, and selenous acid (ITS) preparation have positive effects on chondrocyte proliferation and morphology and investigate the biochemical and histological effects of these additive substances in different cell culture media. Methods: Human cartilage-derived cells (hCDCs) were isolated from the cartilage tissue of a 57-year-old woman diagnosed with gonarthrosis. Tissue samples were cultured in Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640. The cells' chondrogenic activities were observed. After serial passagings, cells were divided into 4 groups at the end of the 6th week. On the 14th day, proliferated cells were examined using an inverted microscope with x4, x10, x20 and x40 magnification and microphotographs were taken. Living cell quantity was determined on the first and 14th days using MTS-ELISA cell proliferation assay. Results: DMEM (without adding ITS premix solution) and RPMI-1640 containing ITS premix solution provide proliferation of the chondrogenic cells. The proliferation and viability of chondrocytes were revealed in this study in the 3rd group (DMEM solution without additives). Conclusion: It is suggested that the culture medium ingredients play crucial roles on chondrogenic proliferation in osteochondral tissue cultures.
dc.identifier.doi10.3944/AOTT.2014.2635
dc.identifier.endpage319
dc.identifier.issn1017-995X
dc.identifier.issue3
dc.identifier.pmid24901923
dc.identifier.scopus2-s2.0-84901784685
dc.identifier.scopusqualityQ2
dc.identifier.startpage313
dc.identifier.trdizinid158868
dc.identifier.urihttps://doi.org/10.3944/AOTT.2014.2635
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/158868
dc.identifier.urihttps://hdl.handle.net/20.500.14704/868
dc.identifier.volume48
dc.identifier.wosWOS:000343288200014
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Assoc Orthopaedics Traumatology
dc.relation.ispartofActa Orthopaedica Et Traumatologica Turcica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250326
dc.subjectCell culture; chondrogenic differentiation; DMEM; ITS premix solution; RPMI-1640
dc.titleDoes insulin, transferrin and selenous acid preparation effect chondrocyte proliferation?
dc.typeArticle

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