Are radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

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dc.authoridOzdemir, Cigdem/0000-0001-8500-0744
dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337
dc.authoridYilmaz, Ibrahim/0000-0003-1503-4046
dc.authoridyasar sirin, duygu/0000-0002-1224-442X
dc.authoridakyuva, yener/0000-0001-8171-5929
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaplan, Necati
dc.contributor.authorÇalışkan, Tezcan
dc.contributor.authorOzdemir, Cigdem
dc.date.accessioned2025-03-26T17:34:57Z
dc.date.available2025-03-26T17:34:57Z
dc.date.issued2019
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractIn the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. In this context, it was aimed to evaluate the effects of iopromide and gadoxetic acid, frequently used in the discography, on intact lumbar disc tissue in pharmaco-molecular and histopathological level. Primary cell cultures were prepared from the healthy disc tissue of the patients operated in the neurosurgery clinic. Except for the control group, the cultures were incubated with the indicated radio-contrast agents. Cell viability, toxicity and proliferation indices were tested at specific time intervals. The cell viability was quantitatively analysed. It was also visually rechecked under a fluorescence microscope with acridine orange/propidium iodide staining. Simultaneously, cell surface morphology was analysed with an inverted light microscope, while haematoxylin and eosin (H&E) staining methodology was used in the histopathological evaluations. The obtained data were evaluated statistically. Unlike the literature, iopromide or gadoxetic acid did not have any adverse effects on the cell viability, proliferation and toxicity (P < 0.05). Although this study reveals that radio-contrast pharmaceuticals used in the discography, often used in neurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.
dc.identifier.doi10.1111/bcpt.13112
dc.identifier.endpage189
dc.identifier.issn1742-7835
dc.identifier.issn1742-7843
dc.identifier.issue2
dc.identifier.pmid30120906
dc.identifier.scopus2-s2.0-85053398131
dc.identifier.scopusqualityQ2
dc.identifier.startpage181
dc.identifier.urihttps://doi.org/10.1111/bcpt.13112
dc.identifier.urihttps://hdl.handle.net/20.500.14704/955
dc.identifier.volume124
dc.identifier.wosWOS:000456596400006
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250326
dc.subjectcytotoxicity; discography; intact intervertebral disc; primary cell culture; radio-contrast agents
dc.titleAre radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?
dc.typeArticle

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