Cloning, expression and characterization of recombinant CagA protein of Helicobacter pylori using monoclonal antibodies: Its potential in diagnostics

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dc.authoridBayyurt, Nizamettin/0000-0001-6993-4715
dc.authoridbolek, bora kazim/0000-0002-1895-1635
dc.authoridUSLU, MERVE/0000-0002-5410-7052
dc.contributor.authorSalih, Barik A.
dc.contributor.authorKarakus, Cebrail
dc.contributor.authorUlupinar, Zeynep
dc.contributor.authorAkbas, Fahri
dc.contributor.authorUslu, Merve
dc.contributor.authorYazici, Duygu
dc.contributor.authorBolek, Bora Kazim
dc.date.accessioned2025-03-26T17:35:00Z
dc.date.available2025-03-26T17:35:00Z
dc.date.issued2020
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractHelicobacter pylori CagA protein plays an important role in the severity of the gastric diseases. Our aims were to clone the cagA 5'conserved region of the gene, characterize the recombinant CagA (rCagA) protein by monoclonal antibodies (mAbs) and to use this protein for the detection of anti-CagA antibodies by an ELISA test. Our developed rCagA protein (67 kDa) showed an amino acid sequence homology of 83% and 80% with Western and East Asian type strains respectively. Two anti-rCagA (BS-53, CK-02) mAbs and 2 additional rCagA proteins of smaller sizes (60 kDa, 28 kDa) were developed for epitope determination. The BS-53 mAb recognized all 3 rCagA proteins while CK-02 mAb recognized only 2 of them indicating recognition of different epitopes. An in-house indirect ELISA using rCagA was developed to detect anti-CagA antibodies in sera of 59 patients. The ELISA results obtained when compared to those of the PCR gave a sensitivity, specificity and accuracy of 81%, 100% and 88% respectively. We have developed for the first time: a rCagA protein that showed high sequence homology with both Western and East Asian type strains and an indirect ELISA of high performance which can be used to detect anti-CagA antibodies in sera of infected patients worldwide.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [111T370]
dc.description.sponsorshipThis study was supported by the grant of the Scientific and Technological Research Council of Turkey (TUBITAK) (No: 111T370). The authors would like to thank Dr. Fatima Yucel, Dr. Esin Akcael, Dr. Hivda Polat and Harun Kocaaga for their technical assistance.
dc.identifier.doi10.1016/j.biologicals.2020.09.002
dc.identifier.endpage31
dc.identifier.issn1045-1056
dc.identifier.issn1095-8320
dc.identifier.pmid32943295
dc.identifier.scopus2-s2.0-85090953397
dc.identifier.scopusqualityQ2
dc.identifier.startpage26
dc.identifier.urihttps://doi.org/10.1016/j.biologicals.2020.09.002
dc.identifier.urihttps://hdl.handle.net/20.500.14704/1002
dc.identifier.volume68
dc.identifier.wosWOS:000594534700005
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.relation.ispartofBiologicals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250326
dc.subjectHelicobacter pylori; Recombinant CagA; Monoclonal antibody; ELISA
dc.titleCloning, expression and characterization of recombinant CagA protein of Helicobacter pylori using monoclonal antibodies: Its potential in diagnostics
dc.typeArticle

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