Effects of cultural conditions on exopolysaccharide production by Bacillus sp. ZBP4

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dc.contributor.authorErgene, Erdi
dc.contributor.authorAvci, Ayşe
dc.date.accessioned2025-03-26T16:10:01Z
dc.date.available2025-03-26T16:10:01Z
dc.date.issued2018
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractMicrobial exopolysaccharides (EPSs) are of great interest for the application in various industries due to their gelling, stabilizing, emulsifying, and antioxidant properties. In the present study, EPS production of 12 Bacillus strains were investigated and the best producer, namely Bacillus sp. ZBP4, was selected for further studies in order to determine the effects of fermentation conditions on the biosynthesis of EPSs. Beet molasses was used as substrate in the experiments. The highest amount of EPS was obtained at 60 g L-1 molasses concentration within 24 h. Optimum temperature and pH were determined as 45oC and 5.0, respectively. Various carbon sources (glucose, starch, lactose, whey, mannitol, sucrose, beet molasses) have been tested for EPS production and beet molasses was found as the best. Using inorganic nitrogen source (ammonium sulfate) caused a decrease in the production of EPS. Tryptone gave the highest EPS yields amongst the organic nitrogen sources (yeast extract, peptone, tryptone) tested. Considerable increase in EPS production (1071 mg L-1) has been observed when the experiment was conducted under the optimized conditions (using tryptone and 60 g L-1 molasses at pH 5.0 and 45 °C in 24 h) which was 143 mg L-1 before the optimization studies. © Ankara Üniversitesi Ziraat Fakültesi.
dc.description.sponsorshipSakarya Üniversitesi, SAU
dc.identifier.doi10.15832/ankutbd.456666
dc.identifier.endpage393
dc.identifier.issn1300-7580
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85053272689
dc.identifier.scopusqualityQ3
dc.identifier.startpage386
dc.identifier.trdizinid286311
dc.identifier.urihttps://doi.org/10.15832/ankutbd.456666
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/286311
dc.identifier.urihttps://hdl.handle.net/20.500.14704/779
dc.identifier.volume24
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherAnkara University
dc.relation.ispartofTarim Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250326
dc.subjectBacillus
dc.subjectBeet molasses
dc.subjectExopolysaccharide (EPS)
dc.titleEffects of cultural conditions on exopolysaccharide production by Bacillus sp. ZBP4
dc.typeArticle

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