Phylogenetic analysis of Helicobacter pylori cagA gene of Turkish isolates and the association with gastric pathology

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dc.authoridYILDIZ, Mehmet Taha/0000-0003-4768-0333
dc.contributor.authorSalih, Barik A.
dc.contributor.authorBolek, Bora Kazim
dc.contributor.authorYildiz, Mehmet Taha
dc.contributor.authorArikan, Soykan
dc.date.accessioned2025-03-26T17:34:56Z
dc.date.available2025-03-26T17:34:56Z
dc.date.issued2013
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractBackground: The cagA gene is one of the important virulence factors of Helicobacter pylori. The diversity of cagA 5' conserved region is thought to reflect the phylogenetic relationships between different H. pylori isolates and their association with peptic ulceration. Significant geographical differences among isolates have been reported. The aim of this study is to compare Turkish H. pylori isolates with isolates from different geographical locations and to correlate the association with peptic ulceration. Methods: Total of 52 isolates of which 19 were Turkish and 33 from other geographic locations were studied. Gastric antral biopsies collected from 19 Turkish patients (Gastritis = 12, ulcer = 7) were used to amplify the cagA 5' region by PCR then followed by DNA sequencing. Results: The phylogenetic tree displayed 3 groups: A) a mix of 2 sub-groups Asian and African/Anatolian/Asian/European, B) Anatolian/European and C) American-Indian. Turkish H. pylori isolates clustered in the mixed sub-group A were mostly from gastritis patients while those clustered in group B were from peptic ulcer patients. A phylogenetic tree constructed for our Turkish isolates detected distinctive features among those from gastritis and ulcer patients. We have found that 2/3 of the gastritis isolates were clustered alone while 1/3 was clustered together with the ulcer isolates. Several amino acids were found to be shared between the later groups but not with the first group of gastritis. Conclusions: This study provided an additional insight into the profile of our cagA gene which implies a relationship in geographic locations of the isolates.
dc.description.sponsorshipFatih University [P50030903_2]; Scientific and Technological Research Council of Turkey (TUBITAK) [111 T370]
dc.description.sponsorshipThis study was supported by the grants of Fatih University (No: P50030903_2) and of the Scientific and Technological Research Council of Turkey (TUBITAK) (No: 111 T370).
dc.identifier.doi10.1186/1757-4749-5-33
dc.identifier.issn1757-4749
dc.identifier.pmid24245965
dc.identifier.scopus2-s2.0-84887767929
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/1757-4749-5-33
dc.identifier.urihttps://hdl.handle.net/20.500.14704/943
dc.identifier.volume5
dc.identifier.wosWOS:000328550400001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBMC
dc.relation.ispartofGut Pathogens
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250326
dc.subjectHelicobacter pylori; Phylogenetic analysis; cagA
dc.titlePhylogenetic analysis of Helicobacter pylori cagA gene of Turkish isolates and the association with gastric pathology
dc.typeArticle

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