The Effect of Ceruloplasmin Against Ischemia-Reperfusion Injury in Epigastric Island Flap in Rats

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dc.contributor.authorSeyid, Mircafer
dc.contributor.authorTiftikcioglu, Yigit
dc.contributor.authorErdem, Mehmet
dc.contributor.authorAkdemir, Övünç
dc.contributor.authorTatar, Burak Ergün
dc.contributor.authorUyanıkgil, Yigit
dc.contributor.authorErcan, Gülinnaz
dc.date.accessioned2025-03-26T16:09:59Z
dc.date.available2025-03-26T16:09:59Z
dc.date.issued2021
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractBackground: Flap surgery is frequently used in plastic surgery to close tissue defects. Ischemia-reperfusion (I/R) injury is a significant problem resulting in partial or total flap necrosis. This study aimed to investigate the effect of ceruloplasmin on I/R injury in epigastric island flaps in rats. Material and method: A total of 32 male Sprague-Dawley rats were divided into four groups with eight rats in each group: The flap was not elevated in Group I; the flap was elevated without ischemia or any application in Group II, after the intraperitoneal saline and ceruloplasmin application the flaps were elevated and ischemia was created in group III-IV, respectively. Bilateral epigastric artery flap was elevated in all groups except Group I. After 6 h of ischemia, the flap was reperfused and inset. Samples were taken from the right and left side of the flap area in other groups at the postoperative 24th h for biochemical analysis (catalase and malondialdehyde-MDA) and the seventh postoperative day for histopathological analysis (Modified Verhofstad score and epidermal thicknesses), respectively. Image analysis for necrosis areas was performed on photos taken on the 7th d. Results: Catalase level was significantly higher in Group IV.(0.15 ± 0.04 U/mg protein) (P < 0.05) Necrosis area percentage(14.4% ± 3.3%),MDA(3.6 ± 0.9 nmol/mg protein), edema(3), necrosis(2.75), and polymorphonuclear leukocyte infiltration(2.87) scores were significantly higher in group III.(P < 0.05). Fibroblast proliferation, collagen density (0.25), vascular density (0.25) scores and epidermal thickness (15.68 µm,) was significantly lower in group III. (P < 0.05) Conclusions: Our study demonstrated that ceruloplasmin application before ischemia reduced I/R injury in epigastric island flaps in rats. © 2021 Elsevier Inc.
dc.identifier.doi10.1016/j.jss.2021.05.045
dc.identifier.endpage635
dc.identifier.issn0022-4804
dc.identifier.pmid34273792
dc.identifier.scopus2-s2.0-85109950008
dc.identifier.scopusqualityQ1
dc.identifier.startpage627
dc.identifier.urihttps://doi.org/10.1016/j.jss.2021.05.045
dc.identifier.urihttps://hdl.handle.net/20.500.14704/748
dc.identifier.volume267
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc.
dc.relation.ispartofJournal of Surgical Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250326
dc.subjectCeruloplasmin
dc.subjectFlap
dc.subjectIschemia-Reperfusion injury
dc.titleThe Effect of Ceruloplasmin Against Ischemia-Reperfusion Injury in Epigastric Island Flap in Rats
dc.typeArticle

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