Determination of Biotin Interference in Pediatric Obesity Related ELISA Research Kits Biotin Interference in Manual ELISA Kits

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dc.contributor.authorKurkcu Kahraman, Ezgi
dc.contributor.authorDonma, Orkide
dc.contributor.authorDonma, Mustafa Metin
dc.contributor.authorYilmaz, Ahsen
dc.contributor.authorGuzel, Savas
dc.date.accessioned2025-07-23T20:44:15Z
dc.date.available2025-07-23T20:44:15Z
dc.date.issued2025
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractBackgroundAlthough high-dose biotin interference in automated immunoassays is now considered, there are very few studies showing biotin interference in manually operated research kits, especially with enzyme-linked immunosorbent assay (ELISA). The aims of our study were to determine the effects of biotin interference on various parameters, including leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, peroxisome proliferator activated receptor gamma (PPAR gamma), nod-like receptor pyrin domain-containing 3 (NLRP3) and interleukin-18 (IL-18), which contribute to energy homeostasis in healthy and obese children.MethodsSerum pools were prepared from healthy and obese individuals, and biotin concentrations in samples containing different amounts of biotin were measured via sandwich and competitive ELISA methods. In addition, possible biotin interactions were investigated by determining the concentrations of all the study parameters in serum pools containing different amounts of biotin.ResultsWe found that the biotin-competitive, ghrelin-competitive, KISS1-competitive, GHSR, leptin and LEPR ELISA kits were less affected by biotin interference and the results of these assay kits were more reliable. Unexpectedly, high levels were also measured in the biotin sandwich ELISA kit, indicating that biotin interference can also occur in manually operated assay kits.ConclusionsBiotin exhibited an interference effect even in well-functioning, qualified kits, and this negative effect was less common in competitive kits. Biotin interference was closely associated with the quality of the research kit, the parameters studied, and the presence of high biotin concentrations in the blood.
dc.description.sponsorshipIstanbul University-Cerrahpasa
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University-Cerrahpasa, Project No: 34574; Beykent University. The funding organization played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
dc.identifier.doi10.1002/jcla.70079
dc.identifier.issn0887-8013
dc.identifier.issn1098-2825
dc.identifier.pmid40657850
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.1002/jcla.70079
dc.identifier.urihttps://hdl.handle.net/20.500.14704/1120
dc.identifier.wosWOS:001527834500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF CLINICAL LABORATORY ANALYSIS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250711
dc.subjectbiotin interference
dc.subjectELISA
dc.subjectpediatric obesity
dc.titleDetermination of Biotin Interference in Pediatric Obesity Related ELISA Research Kits Biotin Interference in Manual ELISA Kits
dc.typeArticle

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