Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?

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dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorBaykız, Derya
dc.contributor.authorDemirkiran, Aykut
dc.contributor.authorAteş, Özkan
dc.date.accessioned2025-03-26T15:54:26Z
dc.date.available2025-03-26T15:54:26Z
dc.date.issued2018
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractAim: Nimodipine is used to prevent cerebrovascular-originated ischemic neurological deficits, yet its effects on nucleus pulposus (NP) cells or annulus fibrosus (AF) cells weren’t studied. This study aimed to examine nimodipine’s effects on vitality and proliferation of chondroadherin (CHAD), type II collagen (COL2A1), and hypoxia-inducible factor 1 alpha (HIF 1?) gene expression in human primary NP/AF cells.Material and Methods: NP/AF cell cultures obtained from 6 patients who underwent microdiscectomy were treated with 100 µMolar nimodipine and analyzed at 0, 24, and 48 h. Data were evaluated using one-way ANOVA and post-hoc Tukey HSD with 95% confidence interval.Results: We observed suppressed cell proliferation and increased necrosis in nimodipine-treated NP/AF cell cultures, especially degenerated tissue. COL2A1 gene expression wasn’t detected in any experimental groups. CHAD and HIF 1? expression had timedependent decreases in control. CHAD and HIF 1? expression were found to decrease at 24h, but increased at 48h in degenerated tissue. In nimodipine-applied intact tissues, CHAD expression was stable at 24h but 1.62 times higher than control at 48h. HIF 1? levels were lower than control.Conclusion: In nimodipine-treated degenerated AF/NP cultures, CHAD and HIF 1? expressions had time-dependent decreases. However, after complete RT-PCR data evaluation, no correlation between nimodipine application and gene expression occurred.
dc.identifier.doi10.5455/jtomc.2018.04.063
dc.identifier.endpage273
dc.identifier.issn2636-7688
dc.identifier.issue2
dc.identifier.startpage266
dc.identifier.trdizinid285556
dc.identifier.urihttps://doi.org/10.5455/jtomc.2018.04.063
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/285556
dc.identifier.urihttps://hdl.handle.net/20.500.14704/516
dc.identifier.volume25
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofAnnals of Medical Research
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR_20250326
dc.subjectGenel ve Dahili Tıp
dc.subjectNörolojik Bilimler
dc.titleDo we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?
dc.typeArticle

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