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    DNA repair gene OGG1 polymorphism and its relation with oxidative DNA damage in patients with Alzheimer's disease
    (Elsevier Ireland Ltd, 2019) Dincer, Yildiz; Akkaya, Caglayan; Mutlu, Tuba; Yavuzer, Serap; Erkol, Gokhan; Bozluolcay, Melda; Guven, Mehmet
    There is considerable evidence that oxidative DNA damage is increased, DNA repair capacity is decreased in patients with Alzheimer's disease. Base excision repair is the major pathway in removal of oxidative DNA damage. 8-oxo-deoxyguanosine DNA glycosylase 1 (OGG1) is the enzyme which is involved in the first step of this repair process. Alterations in DNA repair capacity may be related with polymorphisms in DNA repair genes. In order to investigate the effect of OGG1 Ser326Cys polymorphism on oxidative DNA damage level, OGG1 genotyping was performed, basal and oxidative DNA damage in lymphocytes and 8-OHdG level in plasma were examined in patients with Alzheimer's disease. Basal and oxidative DNA damage and 8-OHdG level were measured by OGG1-modified comet assay and enzyme-linked immunoassay, respectively. OGG1 genotyping was performed by polymerase chain reaction- restriction fragment length polymorphism assay. Basal and oxidative DNA damage and plasma 8-OHdG levels were found to be higher in the Alzheimer's disease group than those in the control group (P < 0.001). In the Alzheimer's disease group, the levels of oxidative DNA damage was higher in the patients having OGG1 (Ser326Cys + Cys326Cys) genotype than those in the patients having OGG1 Ser326Ser genotype. It was concluded that oxidative DNA damage is increased in patients with Alzheimer's disease and OGG1 Ser326Cys polymorphism may be responsible for this increase.
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    The OGG1 Ser326Cys polymorphism and its association with DNA damage and DNA repair in papillary thyroid cancer
    (Hayat Sağlık ve Sosyal Hizmetler Vakfı, 2025) Akkaya Engin, Çağlayan; Yavuzer, Hakan; Teksöz, Serkan; Soylu, Selen; Mete, Meltem; Yavuzer, Serap; Tuz, Ali Ata
    Aim: Hydrogen peroxide, locally produced during thyroid hormone synthesis, leads to oxidative stress in the thyroid gland. Defective repair of oxidative DNA lesions contributes to tumor development. This study aimed to understand the importance of DNA damage and repair on thyroid cancer development through the impact of the DNA repair gene OGG1 Ser326Cys polymorphism that has clinical significance in untreated patients with papillary thyroid cancer. Methods: The study was performed with 70 patients with papillary thyroid cancer and 73 volunteers as control. In lymphocytes, endogenous DNA damage, H2O2-induced DNA damage, and DNA damage after repair were determined by comet assay. The polymerase chain reaction-restriction fragment length polymorphism method was performed for OGG1 genotyping. Results: Endogenous DNA damage, H2O2-induced DNA damage, and DNA damage after repair were higher in patients with thyroid cancer than in the controls (P