Endurance Training and Exogenous Adenosine Infusion Mitigate Hippocampal Inflammation and Cell Death in a Rat Model of Cerebral Ischemia/Reperfusion Injury: A Randomized Controlled Trial

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dc.authoridEslami, Zahra/0000-0002-3187-3977
dc.authoridEGHBAL MOGHANLOU, Abdorreza/0000-0003-1238-0541
dc.contributor.authorEslami, Zahra
dc.contributor.authorGhomi, Masoumeh Rezaei
dc.contributor.authorSaidi, Aref
dc.contributor.authorMousavi, Seyedeh Vafa
dc.contributor.authorFarhadi, Mahboubeh
dc.contributor.authorRobati, Najmeh Sheikh
dc.contributor.authorMoghanlou, Abdorreza Eghbal
dc.date.accessioned2025-03-26T17:34:43Z
dc.date.available2025-03-26T17:34:43Z
dc.date.issued2022
dc.departmentİstanbul Esenyurt Üniversitesi
dc.description.abstractBackground: Cerebral ischemia can cause irreversible structural and functional damages to the brain, especially to the hippocampus. Preconditioning with endurance training and endogenous adenosine infusion may reduce ischemia-associated damages. Objectives: This study aimed to evaluate the effect of preconditioning with endurance training and endogenous adenosine infusion on cell death in the hippocampal CA1 region following ischemia/reperfusion injuries in a rat model. Methods: Male Wistar rats were divided into five groups: (1) control (n = 8); (2) ischemia (n =12); (3) endurance training + ischemia (n = 12); (4) adenosine infusion + ischemia (n =12); and (5) endurance training + adenosine infusion + ischemia (n =12). The rats in the training groups ran on a treadmill five days per week for eight weeks. In the adenosine infusion groups, the rats were injected 0.1 mg/mL/kg of adenosine intraperitoneally. Also, in the ischemic groups, both common carotid arteries were clamped for 45 minutes. Cresyl violet staining and real-time polymerase chain reaction (PCR) assay were used to evaluate cell death and cytokine gene expression, respectively. Results: Based on the present results, treatments, including endurance training + ischemia, adenosine infusion + ischemia, and endurance training + adenosine infusion + ischemia reduced the level of interleukin-6 (IL-6) and glutamate gene expression, respectively, compared to the group of ischemia only. In contrast, the expression of nerve growth factor (NGF) and adenosine receptor (A2A) genes increased by seven, four, and two folds in the endurance training + ischemia, adenosine infusion + ischemia, and endurance training + adenosine infusion + ischemia groups, respectively, compared to the group of ischemia only. Conclusions: Endurance training on a treadmill and exogenous adenosine infusion synergistically diminished cell death and reduced the expression of pro-inflammatory cytokines, while promoting the neurotrophic factor expression. When endurance training and adenosine infusion were used as stimulants before the induction of cerebral ischemia, they significantly reduced cell death.
dc.identifier.doi10.5812/ans.119236
dc.identifier.issn2322-3944
dc.identifier.issn2322-5769
dc.identifier.issue1
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.5812/ans.119236
dc.identifier.urihttps://hdl.handle.net/20.500.14704/839
dc.identifier.volume9
dc.identifier.wosWOS:000776981700009
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherBriefland
dc.relation.ispartofArchives of Neuroscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250326
dc.subjectExercise Preconditioning; Ischemia/Reperfusion; Exogenous Adenosine; Endurance Training
dc.titleEndurance Training and Exogenous Adenosine Infusion Mitigate Hippocampal Inflammation and Cell Death in a Rat Model of Cerebral Ischemia/Reperfusion Injury: A Randomized Controlled Trial
dc.typeArticle

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