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    Chemical Properties and Antioxidant Activity of Different Extracts from Purslane (Portulaca Oleracea L.)
    (Univ Namik Kemal, 2024) Aoudeh, Eyad; Sat, Ihsan Gungor; Binici, Halil İbrahim
    Purslane (Portulaca oleracea L.) has been known as a medical herb with valuable nutritional and pharmacological properties. Herein, water, methanol, and acetone extracts were prepared from two morphologically different purslane samples, thereafter their content of beta-carotene, chlorophyll a and b, total phenolic compounds (TPC), total flavonoids, and total flavonols was evaluated. Additionally, DPPH center dot scavenging activity and ferrous iron (Fe+2) chelating activity of these extracts were also measured. The acetone extract had the highest amounts of beta-carotene (14.16 mg g(-1) DW), chlorophyll a (40.46 mg g(-1) DW), chlorophyll b (9.94 mg g(-1) DW), TPC (51.01 mg GAE g(-1) DW), flavonoids (133.23 mg RE g(-1) DW), and flavonols (46.94 mg RE g(-1) DW). Whereas the lowest values were observed in the water extract, as 0.03 mg g(-1) DW, 0.03 mg g(-1) DW, 0.06 mg g(-1) DW, (34.24 mg GAE g(-1) DW, 10.98 mg RE g(-1) DW, 3.28 mg RE g(-1) DW, respectively. The acetone extract also showed higher DPPH center dot scavenging activity (IC50 = 57.23 mu g mL(-1)) compared to methanol (IC50 = 65.80 mu g mL(-1)) and water (IC50 = 71.47 mu g mL(-1)) extracts. Regarding the ferrous iron (Fe+2) chelating activity, methanol extract exhibited the highest value followed by water and acetone extracts, being 12.78, 10.20 and 6.08%, respectively. A positive correlation was detected between the DPPH center dot scavenging activities of the purslane extracts and their content of TPC, flavonoid, flavonol, beta-carotene, chlorophyll a, and chlorophyll b. On the other hand, there was no significant correlation between chelating activity of the extracts and their content of beta-carotene, chlorophyll a, TPC, flavonoids, flavonols, and DPPH scavenging activity. Purslane extracts could be suggested as a natural antioxidant substance for many pharmaceutical and food applications.
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    Öğe
    Comparison of antioxidant, phenolic profile, melatonin, and volatile compounds of some selected plant samples
    (Wiley, 2024) Binici, Halil İbrahim; Sat, Ihsan Gungor; Yilmaz, Bilal
    It was aimed to examine the antioxidant, phenolic profile, and volatile compound contents of seven different aromatic plant samples (broccoli, yarpuz, walnut leaves, marshmallow, wild clary, harmala, and common yarrow) collected from Adilcevaz district of Bitlis province in T & uuml;rkiye. Gas chromatography-mass spectrometry (GC-MS) device was used for the volatile profile of the plant varieties. The most abundant volatile compounds were generally heptacosane compound that showed anticancer and antimicrobial effects. Piperitone oxide was detected only in yarpuz samples. Phenolic content and antioxidant activity of the plant samples were found to be highly significant (p < .01). When the amounts of total flavonoids were ranked from the largest to the smallest according to plant varieties, it was determined as wild clary > walnut leaves > yarpuz > common yarrow > marshmallow > harmala > broccoli. According to both antioxidant activity methods, wild clary had the lowest IC50 (half-maximal inhibitory concentration) value, i.e. the highest antioxidant activity. The highest amount of epicatechin was determined in yarpuz (898.30 mu g/g) and the lowest amount of epicatechin was determined in wild clary (86.09 mu g/g). No epicatechin was detected in the other four plant samples. Among the samples, melatonin hormone was detected only in common yarrow, harmala, and broccoli and the highest value was determined in common yarrow (3996.27 ng/g). Therefore, it shows that plant samples are rich sources of phytochemicals that can play an important role in preventing the progression of many diseases related to oxidative stress in traditional medicine treatment as functional food sources.