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    The IAP inhibitor GDC-0152 suppresses cell proliferation and induces apoptosis in colon cancer cell lines
    (National Academy of Sciences of Ukraine, 2024) Özdemir, D.; Göksu, S.; Baydas, G.; Tykhomyrov, A.O.; Ağca, C.A.
    Inhibitors of apoptosis (IAPs), a protein family, which regulates various cellular processes, such as proliferation, migration, and cell death. IAPs are commonly overexpressed in various cancers, including colon cancer, making them appealing therapeutic targets. Aim. The present study was aimed to explore the impact of inhibitor of apoptosis protein (IAP) small molecule inhibitor GDC-0152 on colon cancer cells (HCT-116 and HT-29). Methods. The effects of GDC-0152 on colon cancer cells, cell viability, and proliferation were assessed using WST-1 assay and Real Time Cell Analysis System (xCELLigence), while apoptosis was detected using AO/EBr staining and Annexin V/Flow cytometry assays. The expression of XIAP were analyzed using western blotting analysis. Results. It was shown that GDC-0152 can inhibit the proliferation and clonogenic potential of colon cancer cells. We used a viability assay to demonstrate that GDC-0152 inhibited cell growth with IC50 values 28,90 ?M in HCT-116, and 24,32 ?M in HT-29. A wound healing assay showed that GDC-0152 inhibited migration of colon cancer cells. Further validation found that GDC-0152 induced apoptosis in colon cancer cells. Conclusions. Taken together, our results suggest that GDC-0152 suppressed the CC cell proliferation, migration, and induces apoptosis in a concentration-dependent manner. GDC-0152, a IAP inhibitor, can be considered as a valid approach to target cell death in colon cancer. © Publisher PH "Akademperiodyka" of the NAS of Ukraine, 2024.